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  1. LSD: LEAST-SQUARES METHODS TO ESTIMATE RATES AND DATES FROM SERIAL PHYLOGENIES - v0.2 by Thu-Hien TO If you use this software, please cite: “ Fast dating using least-squares criteria and algorithms”, T-H. Some examples: 1) Examples of command line: for rooted tree, constrained mode, and using variances ./lsd -i rootedtree_file -d date_file -c -v for rooted tree, constrained mode, re-estimate the root position around the given root, and non variances ./lsd -i rootedtree_file -d date_file -c -r l for unrooted tree without outgroups, without constraints, estimate the root position, and using variances ./lsd -i unrootedtree_file -d date_file -c -v -r a for unrooted tree with outgroups, constrained mode, using varainces, remove outgroups to obtain the root ./lsd -i unrootedtree_file -d date_file -g outgroup_file -c -v for rooted tree, constrained mode, using variances, and using given rates to estimate dates ./lsd -i rootedtree_file -d date_file -w given_rate_file -c -v for rooted tree, estimating relative dates with date root=0 and date of all leaves=1, using variances, with constraint ./lsd -i tree_file -c -v -a 0 -z 1 2) Examples of input files: Example of Input_tree_file format (newick) (a:0.12,(b:0.3,c:0.5):0.4); ((a:0.12,b:0.3):0.7,c:0.5); Example of Input_date_file format: 3 a 1999 b 2000 c 1780 Example of Outgroup_file format: 2 outgroup1 outgroup2 Example of given_rate_file format: 0.0068 0.0052 3) Output files: .result : contain the estimated rate, root date and the value of the log likelihood function if variances are used.

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